Saturday June 28.

10.00 - 19.00

Registration

17.00 - 17.30

Conference opening. Venue: Law Faculty.

17.30 - 18.30

Biotrans session 1, Opening lecture

Chair: David H.G. Crout (Warwick, GB)

17.30 - 18.30

Romas Kazlauskas (Québec, CAN) Creating enantioselective hydrolases for organic synthesis: combining rational and random methods

19.00 - 20.00

Welcome mixer

09.00 - 10.50

COST D25 Workshop, session 1. Venue: Law Faculty

Chair: Tomáš Hudlický (Gainesville, FL, USA)

09.00 - 09.40

Hans-Peter Meyer (Lonza Ltd, Visp, Switzerland) What have we learned during 20 years of industrial biotransformations?

09.40 - 10.15

Klaus D. Kulbe (Vienna, A) Engineering an entirely enzymatic process for L-ascorbic production from D-glucuronic acid

10.15 - 10.50

Berndt Nidetzky (Graz, A) Glucosyl transfer by saccharide phosphorylases: components of mechanism and applications in (glyco)biotechnology.

10.50 - 11.15

Coffee break

11.15 - 13.10

COST D25 Workshop, session 2. Venue: Law Faculty.

Chair: Ludmila Martínková (Prague, CZ)

11.15 - 11.55

Herfried Griengl (Graz, A) The Importance of Substrate Engineering in Biocatalysis

11.55 - 12.30

Manfred Schneider (Wuppertal, D) Enzyme assisted syntheses: From bioactive molecules to the bioconversion of renewable resources

13.10 – 14.30

Lunch

14.30 - 16.20

COST D25, Workshop, session 3. Venue: Law Faculty.

Chair: Jean Bolte (Clermont-Ferrand, F)

14.30 - 15.00

Pierre Vogel (Lausanne, CH) Asymmetric synthesis of long-chain polyketides based on enzymatic desymmetrization or resolution

15.00 - 15.20

Per Berglund (Stockholm, S) Rational redesign of a lipase into a lyase

15.20 – 15.40

Michael Wolberg (Geleen, NL) Chemoenzymatic synthesis of the chiral side-chain of atorvastatin

15.40 - 16.15

Reinhold Öhrlein (Basel, CH) Chemo-enzymatic approach to statin-side chain building blocks

16.15 - 16.30

Coffee Break

16.30 - 18.30

COST D25 Management Committee meeting. Venue: Law Faculty

19.00

Ballet performance

Monday June 30.

09.00 - 10.10

COST D25, Workshop, session 4. Venue: Jesuits’ Convent. (parallel session I)

Chair: Maria Cantarella (L'Aquila, I)

09.00 – 09.30

Daniela Monti (Milano, I) Production of a fungal a-L-rhamnosidase library and its use for natural glycoside modification

09.30 – 09.50

Christopher Bucke (London, GB) Oligosaccharide and polysaccharide synthesis and modification using enzyme technology

09.50 – 10.10

Inmaculada Robina (Sevilla, Spain) Glycomimetics as selective tools for glycosidases: Pyrrolidine-polyamines as glycosidase inhibitors and cyanodeoxy glycosides as substrates for glycosidases and nitrile hydrating enzymes

10.10 – 10.40

10.40 - 12.00

COST D25, Workshop, session 5. Venue: Jesuits’ Convent. (parallel session I)

Chair: Liisa Kanerva (Turku, SF)

10.40 – 11.00

A. Cavaco-Paulo (Guimaraes, P) Monitoring the biotransformations of acrylic fibres

11.00 – 11.20

G. M. Guebitz (Graz, A) Biotransformation of azo-compounds with new alkalitermostable oxidoreductases

11.20 – 11.40

P.M.L. Lourenço (Lisbon, P) Nitrile hydratase operon from Agrobacterium tumefaciens

11.40 – 12.00

L. Fischer (Stuttgart, D) Enantioselective hydrolysis of mandelonitrile

12.00 – 15.30

15.30 - 17.30

COST D25, Workshop, session 6. Venue: Jesuits’ Convent. (parallel session I)

Chair: Fred van Rantwijk (Delft, NL)

15.30 – 16.00

J.-L. Reymond (Bern, CH) Crystal structure and mutational study of enantioselective protonation catalytic antibody 14D9

16.00 – 16.20

Ulf Hanefeld (Delft, NL) Encapsulation of Hevea brasiliensis hydroxynitrile lyase in sol-gels

16.20 – 16.40

A. C. Pierre (Villeurbanne, F) Silica aerogels as encapsulation media for lipases

16.40 – 17.00

Isabelle Goubet (La Rochelle, F) Multistep catalysis by whole dehydrated cells in a solid-gas bioreactor: Influence of operating parameters on depollution efficiency

17.00 – 17.30

Pere Clapés (Barcelona, E) Enzymatic stereoselective aldol condensations in highly concentrated emulsions (gel emulsions)

09.00 – 10.10

Chair: Antonio Ballesteros (Madrid, E)

09.00 – 09.30

David Guieysse (Toulouse, F) New computational analysis of Burkholderia cepacia lipase enantioselectivity: Modelling of the enantiomer trajectory into the active site.

09.30 – 09.50

Olga Ulbert (Veszprém, H) Development of resolution reactions catalysed by lipases in non-conventional media: Production of chiral carboxylic acids possessing biological activity

09.50 – 10.10

Marco Bocola (Mülheim/Ruhr, D) Learning from directed evolution: Insights in lipase enantioselectivity

10.10 – 10.40

10.40 - 12.00

Biotrans session 3. Venue: Jesuits’ Convent. (parallel session II)

Chair: Zdeněk Wimmer (Prague, CZ)

10.40 – 11.00

Marko D. Mihovilovic (Vienna, A) Enantiodivergent Baeyer-Villiger oxidations by recombinant whole cells

11.00 – 11.20

Uwe Rinner (Gainesville, USA) Benefits of whole cell oxidation of aromatics in manufacturing of fine chemicals and pharmaceuticals

11.20 – 11.40

Zhi Li (Zürich, CH) Regio- and stereoselective biotransformations with Sphingomonas sp. HXN-200

11.40 – 12.00

Radka Chaloupková (Brno, CZ) Safe modification of enzyme substrate specificity by engineering of surface residues in the entrance tunnel

12.00 – 15.30

15.30 - 17.30

Biotrans session 4. Venue: Jesuits’ Convent. (parallel session II)

Chair: Stephanie Burton (Cape Town, SA)

15.30 – 15.50

Vladimir I. Tishkov (Moscow, RU) Formate dehydrogenase: From wild-type enzyme to robust biocatalyst for NAD(P)H regeneration

15.50 – 16.10

Andreas S. Bommarius (Atlanta,USA) Enantioselective oxidation employingADH and NADH oxidase: Cofactor regeneration of both NAD+ from NADH and NADP+ from NADPH

16.10 – 16.30

Andreas Liese (Jülich, D) Molecular hydrogen as efficient reduction equivalent for biocatalysis?

16.30 – 16.50

Bruno Botta (Roma, I) A novel peroxidase-catalyzed reaction. Substrate specificity and kinetic studies

16.50 – 17.10

Friso van Assema (Geleen, NL) Synthesis of optical antipodes of a-amino-b-hydroxyacids using D- and L-threonine aldolases

17.10 – 17.30

Karel Bezouška (Prague, CZ) Structural investigations of fungal b-N-acetylhexosaminidases useful in syntheses of new unique oligosaccharides

17.30 – 19.30

Poster session with wine and cheese, Exhibition Venue: Jesuits’ Convent

Tuesday July 1.

09.00 - 10.50

COST D25, Workshop, session 7. Venue: Law Faculty.

Chair: Vicente Gotor (Oviedo, E) (COST Chairperson)

09.00 - 09.40

Gregg Whited (Palo Alto, CA, USA) Pathway engineering for production of 1,3-propanediol from glucose.

09.40 - 10.15

Patrick Adlercreutz (Lund, S) Influence of water on hydrolases in organic media

10.15 - 10.45

Coffee break

11.15 - 11.50

Biotrans, session 5. Venue: Law Faculty.

Chair: Roger Sheldon (Delft, NL)

10.45 – 11.20

Peter Biely (Bratislava, SK) Acetylation of carbohydrates catalyzed by an acetylxylan esterase in microemulsions

11.20 - 11.55

Wolfgang Kroutil (Graz, A) Biocatalytic Asymmetric Hydrogen Transfer.

12.00 - 13.30

Lunch

13.30 - 20.00

Excursions

Wednesday July 2.

09.00 - 10.50

Biotrans, session 6. Venue: Law Faculty.

Chair: Vytas Svedas (Moscow, Russia)

09.00 - 09.40

Marcell Wubbolts (Geelen, NL) Novel Biocatalyst for the production of enantiomerically pure amines and amino acids

09.40 - 10.15

Nick J. Turner (Edinburgh, GB) Deracemisation of chiral amines using enzymes obtained by directed evolution

10.15 - 10.50

Don A. Cowan (Cape Town, South Africa) Metagenomics, gene dicovery and the "ideal biocatalyst"

10.50 - 11.15

Coffee break

11.15 - 12.30

Biotrans, session 7. Venue: Law Faculty.

Chair: Stefano Servi (Milan, I)

11.15 - 11.55

Takeshi Sugai (Yokohama, J) Complementary and synergistic application of enzyme and molecular catalysis.

11.55 - 12.30

Pawel Kafarski (Wroclaw, PL) Application of bacteria and fungi as biocatalysts for the preparation of optically active hydroxyphosphonates.

12.30 - 14.00

Lunch

14.00 - 16.30

Biotrans, session 8. Venue: Law Faculty.

Chair: Adrie Straathof (Delt, NL)

14.00 – 14.30

Tadashi Ema (Okayama, J) Enantiospecific lipase-catalysed reactions at high temperatures up to 120 ˚C in autoclave

14.30 – 15.00

Andreas Schmid (Zürich, CH) New approaches in electroenzymology: coupling homogeneous and enzyme catalysis for highly specific hydroxylations, epoxidations and hydrogenations

15.00 - 15.30

Peter J. Halling (Glasgow, UK) Equilibrium of reactions on solid-phase substrates can be shifted for useful enzymatic syntheses

15.30 – 16.00

Olivier Favre-Bulle (Nimes, France) Developing novel biocatalysts for manufacturing optically active compounds

16.00 – 16.30

Klaus Liebeton (Zwingenberg, G) New biocatalysts from the metagenome: Alpha/beta hydrolase fold enzymes

17.00 - 18.00

Poster session. Venue: Jesuits’ Convent.

19.00

Conference Dinner.

Thursday July 3.

09.00 - 10.50

Biotrans, session 9. Venue: Law Faculty.

Chair: Wolf-Dieter Fessner (Darmstadt, D)

09.00 - 09.40

Vladimír Maťha (Opava, CZ) Biotransformation of immunosuppressive compounds.

09.40 - 10.15

Karlheinz Drauz  (Hanau, D) Enzymatic Catalysis in Organic Synthesis

10.15 - 10.50

Anke Krebber (Redwood City, USA) Directed Molecular Evolution Technologies: Improving genes, pathways, and whole organisms for biocatalysis, fermentation, and process applications.

10.50 - 11.15

Coffee break

11.15 - 12.30

Biotrans, session 10. Venue: Law Faculty.

Chair: Maurice Franssen (Wageningen, NL)

11.15 - 11.45

Jon D. Stewart (Gainesville, USA) Discovery and development of enzymes for stereoselective redox reactions

11.45 - 12.30

Udo Kragl (Rostock, D) Biocatalysis in ionic liquids

12.30 - 12.45

Announcing of Biotrans’05; Closing ceremony

12.45

Lunch

Departure